# What is a glucose standard curve and why did you need to obtain one for this experiment

Effects of high-intensity exercise on blood glucose in type 2 diabetic patients A single session of continuous high-intensity exercise resulted in 60 minutes of postexercise hyperglycemia, 49 while both a single session of HIT, 50 and a 2-week training program 51 have been shown to improve postprandial glucose control over a hour period following exercise. Students should be able to: The equilibrium constant The law of mass action expresses the relative concentrations of reactants and products in terms of a quantity called Keq. The value of Keq is only affected by temperature.

Changes in concentrations do not affect Keq. Keq must be positive. When Keq is much larger than 1, at equilibrium the concentration of product s is far greater reactants. When Keq is much smaller than 1, at equilibrium the concentration of reactants is much greater than products. However, the concentration of all substances will redistribute and a new equilibrium will be achieve. Beer-Lambert law There is a linear relationship between absorbance and concentration when the law is obeyed.

An equilibrium table is then used to calculate the concentrations of all other species in reaction equation. Five 6" test tubes, five 4" test tubes, pipettes, small beakers, four 10 mL graduated cylinders. Any chemical reaction will eventually proceed to a state of dynamic equilibrium, in which the reactants and products are present in definite concentrations.

In some cases, the equilibrium will be attained only after nearly all of the reagents have been consumed and converted to reaction products. Such a reaction is said to "go to completion. This experiment will investigate the reaction of ferric ions with thiocyanate ions and the condition of equilibrium which results.

The concentration of reactants and products remaining at equilibrium will be related to each other by a mathematical expression involving the equilibrium constant of the reaction. The two reactant ions, which are nearly colorless, react to form a red-colored complex, the ferrithiocyanate ion.

Mixing Solutions in Test Tubes Grasp the top of the test tube firmly but not tightly by curling your index finger and thumb around the neck of the test tube these instructions apply to right-handed individuals with your left hand.

With a rapid motion, slap the bottom of the test tube with the outstretched fingers of your right hand. Your fingers should slide past the bottom of the tube after slapping it. Repeat this motion periodically about once every second.

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• Determination Of Glucose Concentration Using Trinder Method Biology Essay
• Melting and freezing stearic acid

If this is done correctly, a vigorous vortex motion is imparted to the liquid in the tube. To prepare a standard solution, pipet 2 mL of 0. Then, add by pipet 8 mL of a fairly concentrated 0.

The Fe NO3 3 solutions are very corrosive. The total volume of the standard solution however, is five times larger than the initial volume of K SCN solution which was added.

After recording the exact concentration of K SCN listed on the label of the stock solution, determine the concentration of the complex present in the standard.What is a glucose standard curve, and why did you need to obtain one for this experiment?

Did you correctly predict how you would measure the amount of plasma glucose in a patient sample using the glucose standard curve? [PhysioEX Chapter 4 exercise 2] PEX What is a glucose standard curve, and why did you need to obtain one for this experiment?

Did you correctly predict how you would measure the amount of plasma glucose in a patient sample using the glucose standard curve? Your answer. DO NOT include the standard curve since this results section omits the part of the lab that involved creating the standard curve and using it to calculate the concentration of an unknown.

All figures should have appropriate figure legends. Construction of the standard curve: Our gel filtration column has a diameter of mm and a length of mm.

From these values you may calculate the total volume of the column. The void volume, V0, of the column has been experimentally determined to be ml.

10 Starter Experiment Sheet - Analysis of Reducing Sugars You need to prepare the following solution • Fehling’s solution o Solution A – Dissolve g of copper (II) sulphate (CuSO H 2 O) made up to cm 3 in a standard flask.

o Solution B – Dissolve g of sodium potassium tartrate in warm. We will write a custom essay sample on Determination Of Glucose Concentration Using Trinder Method Biology Essay specifically for you for only \$ \$/page. A standard curve was to be drawn from the absorbencies acquired from the spectrophotometer at nm the purposes of the experiment were achieved by obtain dependable.

Basic Lab Skills: β-Galactosidase Induction in Escherichia coli